other:Article Title: C11orf54 promotes DNA repair via blocking CMA-mediated degradation of HIF1A
Article Snippet: Antibody Company (catalog number) Application Primary antibodies ACTB Sigma (A8481) WB, 1:10000 GAPDH Cell Signaling Technology (2118) WB, 1:10000 H3 Cell Signaling Technology (9715) WB, 1:10000 C11orf54 Proteintech (23251-1-AP) WB, 1:4000 IF, 1:400 C11orf54 Invitrogen (PA5-78481) WB, 1:4000 p-H2A.X (Ser139) Cell Signaling Technology (9718) WB, 1:1000 IF, 1:200 PARP Cell Signaling Technology (9532) WB, 1:2000 Caspase3 Cell Signaling Technology (9662) WB, 1:2000 Cleaved -Caspase3 Cell Signaling Technology (9664) WB, 1:1000 Bax Proteintech (50599-2-Ig) WB, 1:2000 Bcl-2 Proteintech (12789-1-AP) WB, 1:2000 ATM Proteintech (27156-1-AP) WB, 1:2000 p-ATM (Ser1981) Cell Signaling Technology (5883) WB, 1:2000 CHK1 Proteintech (25887-1-AP) WB, 1:2000 p-CHK1 (Ser345) Cell Signaling Technology (2348) WB, 1:2000 CHK2 Proteintech (13954-1-AP) WB, 1:2000 p-CHK2 (Thr68) Cell Signaling Technology (2197) WB, 1:2000 Ku70 Cell Signaling Technology (4588) WB, 1:2000 Rad51 Proteintech (14961-1-AP) WB, 1:4000 Rad51 Abcam (ab133534) IF, 1:200 HIF1a Cell Signaling Technology (36169) WB, 1:2000 PKM2 Proteintech (15822-1-AP) WB, 1:5000 HK2 Proteintech (66947-1-Ig) WB, 1:5000 LDHA Proteintech (19987-1-AP) WB, 1:5000 PGAM1 Proteintech (16126-1-AP) WB, 1:5000 PFKP Proteintech (13389-1-AP) WB, 1:5000 G6PD Proteintech (25413-1-AP) WB, 1:3000 p62 Sigma (P0067) WB, 1:10000 LC3B Sigma (L7543) WB, 1:5000 HSC70 Proteintech (10654-1-AP) WB, 1:5000 LAMP2A Abcam (ab125068) WB, 1:2000 Flag-tag Sigma (F1804) IP, 1:1000 RRM2 Proteintech (11661-1-AP) WB, 1:5000 Secondary antibodies Alexa Fluor® 488- AffiniPure goat antirabbit IgG Jackson ImmunoResearch IF, 1:400 Alexa Fluor® 594- AffiniPure goat antirabbit IgG Jackson ImmunoResearch IF, 1:400 VeriBlot for IP Detection Reagent (HRP) Abcam (ab131366) IP, 1:1000 Supplementary Table 3.
Article Title: Deoxyribonuclease 1-like 3 Inhibits Hepatocellular Carcinoma Progression by Inducing Apoptosis and Reprogramming Glucose Metabolism
Article Snippet: Primary antibodies implied for Western blotting and IHC analysis Antibody Company (Cat. No.) Working concentrations DNASE1L3 ThermoFisher (PA5-30006) WB: 1/1000 IHC: 1/200 DNASE1L3 Proteintech (67041-1-Ig) WB: 1/500 IHC: 1/100 Myc tag Abcam(ab18185) IP: 1/100 Bax Abcam(ab32503) WB: 1/1000 IHC: 1/200 Bcl2 Abcam(ab59348) WB: 1/1000 C-Myc Abcam (ab32072) WB: 1/1000 Caspase3 Abcam (ab13847) WB: 1/1000 C-Caspase3 Abcam (ab2302) WB: 1/1000 IHC: 1/200 CyclinD1 Abcam (ab40754) WB: 1/2000 HK2 Proteintech (22029-1-AP) WB: 1/500 IP: 1/100 PKM2 Proteintech (15822-1-AP) WB: 1/500 LDHA Proteintech (66287-1-Ig) WB: 1/1000 PDH Proteintech (18068-1-AP) WB: 1/500 GLUT2 Proteintech (20436-1-AP) WB: 1/500 G6PD Proteintech (66373-1-Ig) WB: 1/500 GAPDH Proteintech (60004-1-Ig) WB: 1/5000 Ki67 Abcam (ab16667) IHC: 1/200 PTPN2 Abcam (ab227916) WB: 1/1000 IP: 1/100 P53 Proteintech (60283-2-Ig) CEBPβ SANTA (sc-56637) WB: 1/1000 IP:1/100 ZNF384 Abcam (ab176689) WB: 1/2000 JAK2 Abcam (ab108596) WB: 1/5000 p-JAK2 Abcam (ab32101) WB: 1/2000 IF: 1/500.
Article Title: ZEB1 enhances Warburg effect to facilitate tumorigenesis and metastasis of HCC by transcriptionally activating PFKM
Article Snippet: Key Resource Table: Table S1 Antibodies for Western blot and IHC Antibody Source Catalog number Application(dilution) ZEB1 Proteintech Cat #21544-1-AP WB (1:1000), IHC (1:200) PFKM Proteintech Cat #55028-1-AP WB (1:1000), IHC (1:200) PFKL Proteintech Cat #15652-1-AP WB (1:1000) PFKP Proteintech Cat #13389-1-AP WB (1:1000) E-Cadherin Proteintech Cat #20874-1-AP WB (1:5000) Actin Proteintech Cat # AC-15 WB (1:5000) HK1 Proteintech Cat # 66974-1-Ig WB (1:1000) ALDOA Proteintech Cat #11217-1-AP WB (1:1000) TPI Proteintech Cat #10713-1-AP WB (1:1000) ENO1 Proteintech Cat #11204-1-AP WB (1:500) GAPDH Proteintech Cat #60004-1-Ig WB (1:5000) G6PD Proteintech Cat #25413-1-AP WB (1:1000) LDHA Proteintech Cat #19987-1-AP WB (1:5000) PCNA Proteintech Cat # 10205-2-AP IHC (1:200) Table S2 Primers used in this study Table S2-1 Primers for over expression plasmid constructions: Gene sequence ZEB1 F: 5-ATGGCGGATGGCCCCAG-3 R: 5-GGCTTCATTTGTCTTTTCTTC-3 ZEB1 (RNAi resistance) F: 5-AGTGGCGGTAGACGGCAACGTA-3 R: 5-CTTATTACGTTGCCGTCTAC-3 PFKM F: 5- AGAGAATTCGGATCC-3 R: 5- CTTCCATGGCTCGAG-3 Table S2-2 Primers for PLL3.7 shRNA constructions: Gene Target sequence shRFP 5-GAAATCCACCCGGGGCGCCC-3 shPFKM#1 5-CCTCCAGAAAGCAGGTAAGATT-3 shZEB1#2 5-GGTAGATGGTAATGTAATAT-3 Table S2-3 Primers for promoter constructions: Name sequence Full length F: 5-GCGTGCTAGCTCGAGATTTGGCCTACAGGCTGTTG-3 R: 5-CGGAATGCCAAGCTTCATGATCCACTCTAGAATTTAAG-3 E2 mut -1 F: 5-AAATAACAAATTTTAGCCTGAAAAAC-3 R: 5-TAAAATTTGTTATTTAAATTTATCTCC-3 E2 mut -2 F: 5-CAGTTCAAATGGAAAATGGCAATG-3 R: 5-ATTTTCCATTTGAACTGCTTACTAC-3 E2 mut -3 F: 5-TTCAAATGACAATGGAGCAGAGAAT-3 R: 5-ATTGTCATTTGAACATTACTTTGC-3 Mut-1 F: 5-GCGTGCTAGCTCGAGGGGATGGATAAAGGAAGATAATAAG-3 R: 5-CGGAATGCCAAGCTTCATGATCCACTCTAGAATTTAAG-3 Mut-2 F: 5-GCGTGCTAGCTCGAGATTTGGCCTACAGGCTGTTG-3 R: 5-CGGAATGCCAAGCTTCTCCTTTCTATCCTCATTGC-3 Mut-3 F: 5-GCGTGCTAGCTCGAG ATTTGGCCTACAGGCTGTTG-3 R: 5-CGGAATGCCAAGCTTGTGTTATGGAATTGTGCTATTG-3 Mut-4 F: 5-GCGTGCTAGCTCGAG ATTTGGCCTACAGGCTGTTG-3 R: 5-CGGAATGCCAAGCTTAAATGTCATGTAATCAAGCCC-3 Mut-5 F: 5-GCGTGCTAGCTCGAG ATTTGGCCTACAGGCTGTTG-3 R: 5-CGGAATGCCAAGCTTCTCAGTCAGGCTCTCCCAC-3 Mut-6 F: 5-GCGTGCTAGCTCGAGATTTGGCCTACAGGCTGTTG-3 R: 5-CGGAATGCCAAGCTTATCAAGATGCCACTAGCTAAAG-3 Mut-7 F: 5-GCGTGCTAGCTCGAGTCCTGCTGTGTCTTAACTG-3 R: 5-CGGAATGCCAAGCTTCATGATCCACTCTAGAATTTAAG-3 Mut-8 F: 5-TCGAATTCTAGAGTGGATC-3 R: 5-AGCTGATCCACTCTAGAAT-3 Mut-9 F: 5-TCGATTAAGACAATGGTCA-3 R: 5-AGCTTTAAGACAATGGTCA-3 Mut-10 F: 5-TCGAGATTCCTGCTGTGTCTTAAC-3 R: 5-AGCTGTTAAGACACAGCAGGAATC-3
Western Blot:Article Title: OTUB2 stabilizes U2AF2 to promote the Warburg effect and tumorigenesis via the AKT/mTOR signaling pathway in non-small cell lung cancer
Article Snippet: .. LDHA ATGGCAACTCTAAAGGATCA GCAACTTGCAGTTCGGGC β-actin GTCATTCCAAATATGAGATGCGT GCATTACATAATTTACACGAAAGCA SiRNA used in this study OTUB2-siRNA1 ACCACATCGTGCAGTTCCT OTUB2-siRNA2 TGTGACATTCTATCCATTC U2AF2-siRNA1 GTGAGTACGTGGACATCAA U2AF2-siRNA2 CCAACTACCTGAACGATGA Supplementary Table 2 Antibodies for western blot used in this study Name Company Catalog Number Assay OTUB2 Sangon Biotech #D199590 WB OTUB2 Novus #H00078990-M14 IP U2AF2 Proteintech #15624 WB U2AF2 Abcam #ab37530 IP PFKM Proteintech #55028 WB LDHA Proteintech #19987 WB HIF1A Proteintech #20960 WB GLTU1 Proteintech #55031 WB HK2 Proteintech #22029 WB PGK1 Proteintech #17811 WB PGAM1 Proteintech #16126 WB PKM2 Proteintech #15822 WB ENO1 Proteintech #11204 WB GPI Proteintech #15171 WB c-MYC Proteintech #10828 WB ALDOA Proteintech #11217 WB GAPDH Proteintech #60004 WB mTOR Cell Signaling Technology #2983 WB P-mTOR(ser2481) Cell Signaling Technology #2971 WB AKT Cell Signaling Technology #4691 WB P-AKT(ser473) Cell Signaling Technology #4060 WB β-actin Sigma-aldrich #A3854 WB Ubiquitin Abcam #EPR8589 WB Ubiquitin,Lys48-Specific Millipore #05-1307 WB Ubiquitin,Lys63-Specific Millipore #05–1313 WB HA Santa Cruz #sc-7392 WB Supplementary Table 3 List of possible OTUB2-interacting proteins identified by Mass Spectrometry Protein Name Organism Species Gene Name Unique Peptides Peptid es 60S ribosomal protein L3 Homo sapiens RPL3 11 62 E3 ubiquitin-protein ligase TRIM21 Homo sapiens TRIM21 7 27 Elongation factor 1-alpha 1 Homo sapiens EEF1A1 7 24 tRNA-splicing ligase RtcB Homo sapiens RTCB 6 21 Vimentin Homo sapiens Vim 6 20 Polymerase delta-interacting protein 3 Homo sapiens POLDIP3 6 10 Plasminogen activator inhibitor 1 RNA-binding protein Homo sapiens SERBP1 3 11 Heterogeneous nuclear ribonucleoprotein F Homo sapiens HNRNPF 3 8 Probable ATP-dependent RNA helicase DDX28 Homo sapiens DDX28 3 7 Polypyrimidine tract-binding protein 1 Homo sapiens PTBP1 3 6 Eukaryotic initiation factor 4A-III Homo sapiens EIF4A3 3 5 RCC2 protein Homo sapiens RCC2 2 5 Splicing factor U2AF 65 kDa Homo sapiens U2AF2 2 4 Pre-mRNA-processing factor 19 Homo sapiens PRPF19 2 3 Pyruvate kinase PKM Homo sapiens PKM 2 3 Zinc finger protein 211 Homo sapiens ZNF211 1 6 Transcription factor SOX-10 Homo sapiens SOX10 1 4 Glyceraldehyde-3-phosphate dehydrogenase Homo sapiens GAPDH 1 3 Nuclear receptor coactivator 5 Homo sapiens NCOA5 1 3 Supplementary Table 4 Chi square test of the correlation between OTUB2 and U2AF2 OTUB2-high OTUB2-low P U2AF2-high 15 12 0.043 U2AF2-low 10 23 Supplementary Table 5 Variables predictive of survival by COX proportional hazards model in NSCLC patients Parametes Wald χ 2 Risk Ratio P OTUB2 2.911 1.362 0.088 U2AF2 1.601 1.319 0.206 PGK1 1.668 1.551 0.197 ..
Article Title: UBE2S promotes glycolysis in hepatocellular carcinoma by enhancing E3 enzyme-independent polyubiquitination of VHL
Article Snippet: .. E2F2 Abcam (ab235837) IHC: 1/200 PFKL Abcam (ab181064) WB: 1/1000 HK2 Cell signaling technology (2867) WB: 1/1000 HIF-1α Cell signaling technology (36169) WB: 1/1000, IHC: 1/800 Myc-Tag Cell signaling technology (2276) WB: 1/1000 HA-Tag Cell signaling technology (3724) WB: 1/1000 Flag-Tag Cell signaling technology (8146) WB: 1/1000 Flag-Tag Cell signaling technology (14793) ChIP: 1/50 VHL GeneTex (GTX101087) WB: 1/1000, IHC: 1/500 VHL Santa Cruz (sc-17780) IF: 1/100 UBE2S Biorbyt (orb5098) IF: 1/200 PCNA Sigma-Aldrich (CBL407) IHC: 1/250 UBE2S Proteintech (14115-1-AP) WB: 1/1000, IHC: 1/500 α-tubulin Proteintech (666031-1-Ig) WB: 1/2000 GLUT1 Proteintech (66290-1-Ig) WB: 1/500 LDHA Proteintech (19987-1-AP) WB: 1/2000 VHL Proteintech (24756-1-AP) WB: 1/1000 Ub Proteintech (10201-2-AP) WB: 1/2000 ..
Ubiquitin Proteomics:Article Title: OTUB2 stabilizes U2AF2 to promote the Warburg effect and tumorigenesis via the AKT/mTOR signaling pathway in non-small cell lung cancer
Article Snippet: .. LDHA ATGGCAACTCTAAAGGATCA GCAACTTGCAGTTCGGGC β-actin GTCATTCCAAATATGAGATGCGT GCATTACATAATTTACACGAAAGCA SiRNA used in this study OTUB2-siRNA1 ACCACATCGTGCAGTTCCT OTUB2-siRNA2 TGTGACATTCTATCCATTC U2AF2-siRNA1 GTGAGTACGTGGACATCAA U2AF2-siRNA2 CCAACTACCTGAACGATGA Supplementary Table 2 Antibodies for western blot used in this study Name Company Catalog Number Assay OTUB2 Sangon Biotech #D199590 WB OTUB2 Novus #H00078990-M14 IP U2AF2 Proteintech #15624 WB U2AF2 Abcam #ab37530 IP PFKM Proteintech #55028 WB LDHA Proteintech #19987 WB HIF1A Proteintech #20960 WB GLTU1 Proteintech #55031 WB HK2 Proteintech #22029 WB PGK1 Proteintech #17811 WB PGAM1 Proteintech #16126 WB PKM2 Proteintech #15822 WB ENO1 Proteintech #11204 WB GPI Proteintech #15171 WB c-MYC Proteintech #10828 WB ALDOA Proteintech #11217 WB GAPDH Proteintech #60004 WB mTOR Cell Signaling Technology #2983 WB P-mTOR(ser2481) Cell Signaling Technology #2971 WB AKT Cell Signaling Technology #4691 WB P-AKT(ser473) Cell Signaling Technology #4060 WB β-actin Sigma-aldrich #A3854 WB Ubiquitin Abcam #EPR8589 WB Ubiquitin,Lys48-Specific Millipore #05-1307 WB Ubiquitin,Lys63-Specific Millipore #05–1313 WB HA Santa Cruz #sc-7392 WB Supplementary Table 3 List of possible OTUB2-interacting proteins identified by Mass Spectrometry Protein Name Organism Species Gene Name Unique Peptides Peptid es 60S ribosomal protein L3 Homo sapiens RPL3 11 62 E3 ubiquitin-protein ligase TRIM21 Homo sapiens TRIM21 7 27 Elongation factor 1-alpha 1 Homo sapiens EEF1A1 7 24 tRNA-splicing ligase RtcB Homo sapiens RTCB 6 21 Vimentin Homo sapiens Vim 6 20 Polymerase delta-interacting protein 3 Homo sapiens POLDIP3 6 10 Plasminogen activator inhibitor 1 RNA-binding protein Homo sapiens SERBP1 3 11 Heterogeneous nuclear ribonucleoprotein F Homo sapiens HNRNPF 3 8 Probable ATP-dependent RNA helicase DDX28 Homo sapiens DDX28 3 7 Polypyrimidine tract-binding protein 1 Homo sapiens PTBP1 3 6 Eukaryotic initiation factor 4A-III Homo sapiens EIF4A3 3 5 RCC2 protein Homo sapiens RCC2 2 5 Splicing factor U2AF 65 kDa Homo sapiens U2AF2 2 4 Pre-mRNA-processing factor 19 Homo sapiens PRPF19 2 3 Pyruvate kinase PKM Homo sapiens PKM 2 3 Zinc finger protein 211 Homo sapiens ZNF211 1 6 Transcription factor SOX-10 Homo sapiens SOX10 1 4 Glyceraldehyde-3-phosphate dehydrogenase Homo sapiens GAPDH 1 3 Nuclear receptor coactivator 5 Homo sapiens NCOA5 1 3 Supplementary Table 4 Chi square test of the correlation between OTUB2 and U2AF2 OTUB2-high OTUB2-low P U2AF2-high 15 12 0.043 U2AF2-low 10 23 Supplementary Table 5 Variables predictive of survival by COX proportional hazards model in NSCLC patients Parametes Wald χ 2 Risk Ratio P OTUB2 2.911 1.362 0.088 U2AF2 1.601 1.319 0.206 PGK1 1.668 1.551 0.197 ..
Mass Spectrometry:Article Title: OTUB2 stabilizes U2AF2 to promote the Warburg effect and tumorigenesis via the AKT/mTOR signaling pathway in non-small cell lung cancer
Article Snippet: .. LDHA ATGGCAACTCTAAAGGATCA GCAACTTGCAGTTCGGGC β-actin GTCATTCCAAATATGAGATGCGT GCATTACATAATTTACACGAAAGCA SiRNA used in this study OTUB2-siRNA1 ACCACATCGTGCAGTTCCT OTUB2-siRNA2 TGTGACATTCTATCCATTC U2AF2-siRNA1 GTGAGTACGTGGACATCAA U2AF2-siRNA2 CCAACTACCTGAACGATGA Supplementary Table 2 Antibodies for western blot used in this study Name Company Catalog Number Assay OTUB2 Sangon Biotech #D199590 WB OTUB2 Novus #H00078990-M14 IP U2AF2 Proteintech #15624 WB U2AF2 Abcam #ab37530 IP PFKM Proteintech #55028 WB LDHA Proteintech #19987 WB HIF1A Proteintech #20960 WB GLTU1 Proteintech #55031 WB HK2 Proteintech #22029 WB PGK1 Proteintech #17811 WB PGAM1 Proteintech #16126 WB PKM2 Proteintech #15822 WB ENO1 Proteintech #11204 WB GPI Proteintech #15171 WB c-MYC Proteintech #10828 WB ALDOA Proteintech #11217 WB GAPDH Proteintech #60004 WB mTOR Cell Signaling Technology #2983 WB P-mTOR(ser2481) Cell Signaling Technology #2971 WB AKT Cell Signaling Technology #4691 WB P-AKT(ser473) Cell Signaling Technology #4060 WB β-actin Sigma-aldrich #A3854 WB Ubiquitin Abcam #EPR8589 WB Ubiquitin,Lys48-Specific Millipore #05-1307 WB Ubiquitin,Lys63-Specific Millipore #05–1313 WB HA Santa Cruz #sc-7392 WB Supplementary Table 3 List of possible OTUB2-interacting proteins identified by Mass Spectrometry Protein Name Organism Species Gene Name Unique Peptides Peptid es 60S ribosomal protein L3 Homo sapiens RPL3 11 62 E3 ubiquitin-protein ligase TRIM21 Homo sapiens TRIM21 7 27 Elongation factor 1-alpha 1 Homo sapiens EEF1A1 7 24 tRNA-splicing ligase RtcB Homo sapiens RTCB 6 21 Vimentin Homo sapiens Vim 6 20 Polymerase delta-interacting protein 3 Homo sapiens POLDIP3 6 10 Plasminogen activator inhibitor 1 RNA-binding protein Homo sapiens SERBP1 3 11 Heterogeneous nuclear ribonucleoprotein F Homo sapiens HNRNPF 3 8 Probable ATP-dependent RNA helicase DDX28 Homo sapiens DDX28 3 7 Polypyrimidine tract-binding protein 1 Homo sapiens PTBP1 3 6 Eukaryotic initiation factor 4A-III Homo sapiens EIF4A3 3 5 RCC2 protein Homo sapiens RCC2 2 5 Splicing factor U2AF 65 kDa Homo sapiens U2AF2 2 4 Pre-mRNA-processing factor 19 Homo sapiens PRPF19 2 3 Pyruvate kinase PKM Homo sapiens PKM 2 3 Zinc finger protein 211 Homo sapiens ZNF211 1 6 Transcription factor SOX-10 Homo sapiens SOX10 1 4 Glyceraldehyde-3-phosphate dehydrogenase Homo sapiens GAPDH 1 3 Nuclear receptor coactivator 5 Homo sapiens NCOA5 1 3 Supplementary Table 4 Chi square test of the correlation between OTUB2 and U2AF2 OTUB2-high OTUB2-low P U2AF2-high 15 12 0.043 U2AF2-low 10 23 Supplementary Table 5 Variables predictive of survival by COX proportional hazards model in NSCLC patients Parametes Wald χ 2 Risk Ratio P OTUB2 2.911 1.362 0.088 U2AF2 1.601 1.319 0.206 PGK1 1.668 1.551 0.197 ..
Immunohistochemistry:Article Title: UBE2S promotes glycolysis in hepatocellular carcinoma by enhancing E3 enzyme-independent polyubiquitination of VHL
Article Snippet: .. E2F2 Abcam (ab235837) IHC: 1/200 PFKL Abcam (ab181064) WB: 1/1000 HK2 Cell signaling technology (2867) WB: 1/1000 HIF-1α Cell signaling technology (36169) WB: 1/1000, IHC: 1/800 Myc-Tag Cell signaling technology (2276) WB: 1/1000 HA-Tag Cell signaling technology (3724) WB: 1/1000 Flag-Tag Cell signaling technology (8146) WB: 1/1000 Flag-Tag Cell signaling technology (14793) ChIP: 1/50 VHL GeneTex (GTX101087) WB: 1/1000, IHC: 1/500 VHL Santa Cruz (sc-17780) IF: 1/100 UBE2S Biorbyt (orb5098) IF: 1/200 PCNA Sigma-Aldrich (CBL407) IHC: 1/250 UBE2S Proteintech (14115-1-AP) WB: 1/1000, IHC: 1/500 α-tubulin Proteintech (666031-1-Ig) WB: 1/2000 GLUT1 Proteintech (66290-1-Ig) WB: 1/500 LDHA Proteintech (19987-1-AP) WB: 1/2000 VHL Proteintech (24756-1-AP) WB: 1/1000 Ub Proteintech (10201-2-AP) WB: 1/2000 ..
Chromatin Immunoprecipitation:Article Title: UBE2S promotes glycolysis in hepatocellular carcinoma by enhancing E3 enzyme-independent polyubiquitination of VHL
Article Snippet: .. E2F2 Abcam (ab235837) IHC: 1/200 PFKL Abcam (ab181064) WB: 1/1000 HK2 Cell signaling technology (2867) WB: 1/1000 HIF-1α Cell signaling technology (36169) WB: 1/1000, IHC: 1/800 Myc-Tag Cell signaling technology (2276) WB: 1/1000 HA-Tag Cell signaling technology (3724) WB: 1/1000 Flag-Tag Cell signaling technology (8146) WB: 1/1000 Flag-Tag Cell signaling technology (14793) ChIP: 1/50 VHL GeneTex (GTX101087) WB: 1/1000, IHC: 1/500 VHL Santa Cruz (sc-17780) IF: 1/100 UBE2S Biorbyt (orb5098) IF: 1/200 PCNA Sigma-Aldrich (CBL407) IHC: 1/250 UBE2S Proteintech (14115-1-AP) WB: 1/1000, IHC: 1/500 α-tubulin Proteintech (666031-1-Ig) WB: 1/2000 GLUT1 Proteintech (66290-1-Ig) WB: 1/500 LDHA Proteintech (19987-1-AP) WB: 1/2000 VHL Proteintech (24756-1-AP) WB: 1/1000 Ub Proteintech (10201-2-AP) WB: 1/2000 ..
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